Biotechnology Paper Analysis

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This essay gives a summary of the article, Transcript Profile of Flowering Regulatory Genes in VcFT-Overexpressing Blueberry Plants published in PloS One on June 7, 2016. The authors of the article are Aaron Walworth, Chai Benli, and Song Guo-qing and aimed at offering a transcript profile of flowering regulation genes in VcFT-overexpressing blueberry plants. Plenty of antioxidants in blueberry make them very important for human fitness. According to Walworth et al., there is a need to develop “new cultivars with distinct flowering times and chilling requirements, high cold/heat tolerances, and excessive yields” as top priorities for sustainable blueberry production in mild of climate changes. The article cites the significant amplify in freeze tolerance in floral buds and leaves to show the potential for genetic and genomics transformation in blueberry breeding. The role of flowering genes and environmental signals in flowering is highlighted in the article hence critical for sustainable blueberry production. The study sought to “identify flowering-related gene networks of high bush blueberry” (Walworth et al.).
The research utilized Trinity to develop a transcription reference of 254,396 transcripts and 179,853 gene contigs developed by assembling 72.7 million reads. From the transcriptome reference and querying flowering pathway genes of herbaceous plants, the researchers identified flowering patterns of blueberry. The research findings include the overexpressing of VcFT by the VcFT-Aurora is driven by cauliflower mosaic virus resulting in dwarf plants, continuous flowering, and early floral formation. The overexpression of VcFT promotes early flowering but is not sufficient to substitute the vernalization of blueberries for normal flowering under non-chilling conditions. The research results also depict Trinity as an efficient platform for generating of transcriptome reference for tetraploid blueberry plants (Walworth et al.). The reliability of refTrinity as a reliable transcription reference for identifying blueberry flowering was validated by the finding of orthologues of flowering pathway genes in common in rice, Arabidopsis and cereal crops in blueberry. The analysis of the leaf, flower, and bud differential expression show that expression of flowering-related genes was tissue specific in blueberry and RNA-seq transcriptome offered an efficient approach for confirming gene expression specificity.
The article concludes that the research used RNA sequencing data and Trinity to develop a transcription reference of high bush blueberry. The research developed a flowering-related genes reference for blueberry using the known sequences of the flowering pathway of Arabidopsis, cereal, and rice. The research also concludes that herbaceous plant and blueberry conserved VcFT-mediated flowering through downstream genes including VcSOC1, VcLFY, VcAP1, and VcFUL. The major integrators in VcFT overexpressing plant leaf tissues in photoperiod and vernalization pathway are VcCOL5 and VcMAF2.Vc MAFF5 respectively.
The article is well presented and offers comprehensive information that is well ordered for effective reading and understanding by the readers. The piece depicts the important role biotechnology plays in the current age and provides a platform to overcome and prepare for climate change by ensuring sustainable production of blueberries. The article articulates the use of biotechnology tools and comparative genomics in investigating the flowering pathway genes and flowering mechanisms of blueberry. The article is well organized and efficiently utilizes previous research in the development of the theme and meeting the objectives of the study. The report also achieves its goals and objectives well and provides insightful information on gene flowering regulation in blueberry. The presentation is very effective especially incorporation of visual displays completing the research finding and improving understanding by simplifying concepts.

Work Cited

Walworth, Aaron E., Benli Chai, and Guo-qing Song. "Transcript profile of flowering regulatory genes in VcFT-overexpressing blueberry plants." PLoS one 11.6 (2016): e0156993.

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