Fungi Culture and Bacteria

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Bacteria and fungi have a vary of culturing requirements. These environments vary with the type of organisms. The distinction occurs due to the varying traits of the two organisms. In culturing the two microorganisms, the knowledge of the medium composition is paramount eschew the cases of limiting conditions. A giant number of microbial genera dwell in an environment which satisfactory favors immense proliferation and survival of their various existence forms. In this context, the best environment for these microbial lifestyles forms is an environment with the right temperature, pH, oxygen, moisture and energy requirements.

Results

a) Highest bacterial growth in Saliva.
b) Small growth in aquatic environment.
c) Small growth in hand.

Discussion

Bacterial and fungal microorganisms respond differently to the various environments. However, both require certain conditions which general favor their proliferation and survival. For the case of the of the hand as a n environment, it provides a favorable temperature and oxygen which favors the survival of most bacteria. Additionally, it avails optimum moisture content. Moisture content controls the extent of water activity within the organisms. Correct water activity helps in easy intake of the energy substrates available for microbial survival. The limiting factor in the hand environment is the energy content. There is low access to food which is a limiting factor.
There was a slight growth in aquatic conditions. This is due to the fact that, aquatic pH is favorable to most microbial lives since it is almost neutral (Rappe, Giovinni 363). Additionally, there is sufficient moisture which helps the microorganisms in food intake and excretion. However, there are cases of limiting factors present in the aquatic environment. First, there is high carbon content present in water (Glud, Middelboe 287). This this poses a limitation to proliferation of aerobic life forms. High carbon content limits the penetration of oxygen which is an essential factor for growth and proliferation (Rappe, Giovinni 368). Secondly, the aquatic environment has a low amount of energy. Hence, the microorganisms do not receive a sufficient amount of food responsible for growth and proliferation (Glud, Middelboe 287)..
There was a high bacterial growth due to a number of favoring factors. First, there is a favorable moisture content. This favors the exchange of nutrients, optimum metabolic reactions and removal of waste products to avoid toxicity. Second, there is a favorable pH in saliva. It ranges between 6.5 to 7.5. this pH is conducive for the normal metabolic reactions and proliferation within these microorganisms.
Saliva also acts as a buffer which contains minerals like calcium and phosphates which are favorable for the growth and proliferation of bacteria colonies. Furthermore, the temperature of saliva is almost 37 degrees Celsius (Rappe, Giovinni 368). Microorganisms have an ability to survive within this range. This favored optimum conditions for metabolism. Moreover, there is an optimum access to nutrients (Rappe, Giovinni 365). This is possibly through endogenous nutritional supply. This happens when the microorganisms breakdown the crevicular fluid present in saliva to obtain food which is then absorbed through metabolic exchange. For example, saliva contains sacarose which microorganisms use to make polysaccharides which is used as sources of energy for their survival (Glud, Middelboe 287).

Conclusion

Each factor is equally important in survival and growth of any microorganism. The environment with the most favorable conditions provides a good growth site for most fungi and bacteria (Rappe, Giovinni 368). Other factors like osmotic stress can also impede the growth of most fungi and bacterial since it affects there feeding and excretion abilities result to possible starvation and waste toxicity respectively. Therefore also extreme conditions of these favoring factors can lead to limiting or no growth of these microorganisms (Rappe, Giovinni 368). Therefore an optimum state of the culture medium provides the most favorable environment.

Works Cited.

Glud, Middelboe. “High Rates of Microbial Carbon Turnover sediments in deep Oceanic Trench.” Nature Geoscience. New York, 2013. 6(13): 281-288.

Rappe, Giovinni. “ The Uncultured Microbial Majority.” Annual Review of Microbiology. Wayback, 2003.53(12): 35-372.

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