About Bacteriuria
It refers to the presence in urine or the urinary tract of human beings of pathogenic microorganisms. However, the case of Bacteriuria is not considered when the urine sample is infected in the urine sample. By colonizing the cells, the bacterial infection acts. The physician needs information about the density and pollution of the contaminated bacteria. Compared with that of polluted bacteria, the density of infected bacteria is high. Bacteriuria is considerably significant when the pathogenic number of the microorganisms is more than 100000 colonies per milliliter of the sample, the symptoms are evident, and the infection has reached the kidney. Germs invade and spread within the urinary system by two principal routes. The hematogenous path involves the contamination of the renal parenchyma by the blood-borne organisms in humans. The bacteria are then spread to the urinary tract through the bloodstream where the pathogen find its way into the renal parenchyma and drained into the bladder of the male. The presence of a collection of pus build up within the kidney tissue is noted for patients infected by this route, though not frequent.
The ascending route is mostly associated with females and develops when bacteria from the fecal flora subdue the vaginal opening and dislodge the common microorganism. Colonization of the canal with E. coli is one of the vital steps in the medication of the acute and the recurrent urinary tract infection. Most of the bacteria instigate in the rectal flora and cross the threshold through the blood via the urethra. The urethra is consequently colonized, and the urapathogens gains entry into the bladder through the renal parenchyma that is brought about by urethral massage accompanied in sexual intercourse.
Aseptic urine collection requires careful cleansing of the urogenital surfaces by use of a sponge moistened with tap water. Collection of the sample is important so as to make sure that the microorganism observed collected through the process came from the urinary tract and not the collection container. In males, sponging off the male genitalia is preceded with the discard of the first stream of the urine. The sample is free from external microorganisms when the middle stream of the urine sample is collected. In females, the labial surfaces are cleaned and the container held in a manner that it does not come in contact with the skin so as to avoid the possible contamination of the model from the microorganisms on the skin of the human.
Bacteria species are known to cause the urinary tract infection. They involve Enterobacter, Klebsiella, Serratia, Escherichia coli and the Staphylococcus saprophyticus. These organisms are the genus of gram-negative, facultative anaerobic and are mostly rod-shaped with the exception of the Staphylococcus saprophyticus that is round.
The 20 colonies are contained in the 0.01 milliliter of the urine sample, which is diluted to get a molar concentration of 0.1M. The molar concentration is derived from the ratio of dilution. The capacity of the urine in single molar is calculated by dividing the number of the colonies in the sample by the capacity of the sample in liters (1× 10-5). The calculation brings the number of colonies to 20 million. The number depicts a significant amount of the colonies as the standard number is at 100000 colonies.
Reference
Lab Manual and Workbook in Microbiology: Applications to Patient Care, 11th Edition.
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